1XKI
Crystal structure of human tear lipocalin/von Ebners gland protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97960, 0.97888, 0.88556, 0.97912 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.397, 61.415, 50.798 |
| Unit cell angles | 90.00, 129.71, 90.00 |
Refinement procedure
| Resolution | 39.200 - 1.800 |
| R-factor | 0.19277 |
| Rwork | 0.189 |
| R-free | 0.25420 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.215 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.200 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 16397 | |
| <I/σ(I)> | 5.0843 | 2.54 |
| Completeness [%] | 97.9 | 98.77 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 285 | Tris/HCl, Zn-acetate, ethylene glycol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
