1XK8
Divalent cation tolerant protein CUTA from Homo sapiens O60888
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.157, 89.517, 125.847 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.670 - 2.700 |
| R-factor | 0.24664 |
| Rwork | 0.245 |
| R-free | 0.28316 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1osc |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.048 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
| Rmerge | 0.073 | 0.046 | 0.320 |
| Number of reflections | 24132 | ||
| Completeness [%] | 99.1 | 26.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | modified microbatch | 8.6 | 291 | 1.5M potassium hydrogen phosphate, 0.1M TRIS, pH 8.6, modified microbatch, temperature 291K |
