1XJX
The crystal structures of the DNA binding sites of the RUNX1 transcription factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 |
| Unit cell lengths | 41.032, 41.032, 23.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.700 |
| R-factor | 0.1658 |
| Rwork | 0.162 |
| R-free | 0.25060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | FIBER A-DNA |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.025 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | ULTIMA |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.400 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 4315 | |
| <I/σ(I)> | 39.3 | 4.9 |
| Completeness [%] | 95.9 | 78.4 |
| Redundancy | 3.3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | MPD, magnesium chloride, sodium cacodylate acid, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | magnesium chloride | ||
| 3 | 1 | 1 | sodium cacodylate acid | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | MPD | ||
| 6 | 1 | 2 | magnesium chloride | ||
| 7 | 1 | 2 | sodium cacodylate acid | ||
| 8 | 1 | 2 | H2O |
