1XJV

Crystal structure of human POT1 bound to telomeric single-stranded DNA (TTAGGGTTAG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	160
Detector technology	CCD
Collection date	2003-12-23
Detector	ADSC QUANTUM 4
Wavelength(s)	1.107
Spacegroup name	C 2 2 21
Unit cell lengths	102.026, 103.243, 71.664
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.290 - 1.730
R-factor	0.232
Rwork	0.228
R-free	0.23700
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.390
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.290	1.790
High resolution limit [Å]	1.730	1.730
Number of reflections	38238
<I/σ(I)>	15.2
Completeness [%]	95.4	77.2
Redundancy	7.1	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	289	Ammonium sulfate, DTT, Sodium Chloride, Trisodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	5.6	289	Ammonium sulfate, DTT, Sodium Chloride, Trisodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	5.6	289	Ammonium sulfate, DTT, Sodium Chloride, Trisodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	(NH4)2SO4
2	1	1	NaCl
3	1	1	sodium citrate
4	1	1	DTT
5	1	2	(NH4)2SO4
6	1	2	NaCl
7	1	2	sodium citrate
8	1	2	DTT