1XJR
The Structure of a Rigorously Conserved RNA Element Within the SARS Virus Genome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 93.218, 93.218, 128.109 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 80.850 - 2.700 |
| R-factor | 0.2325 |
| Rwork | 0.231 |
| R-free | 0.24326 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.924 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.850 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.056 | 0.465 |
| Number of reflections | 9497 | |
| <I/σ(I)> | 31.3 | 6.5 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 13.4 | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 4.5 mg/ml s2m RNA in 30 mM Tris, pH 7.6, 100 mM NaCl and 60 mM MgCl2 and 50 mM MES, pH 5.6, 100 mM Mg(OAc)-2 and 20% MPD , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Tris | ||
| 10 | 1 | 2 | MPD | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | MES | ||
| 5 | 1 | 1 | Mg(OAc)-2 | ||
| 6 | 1 | 1 | MPD | ||
| 7 | 1 | 2 | NaCl | ||
| 8 | 1 | 2 | MgCl2 | ||
| 9 | 1 | 2 | MES |
