1XJK
Structural mechanism of allosteric substrate specificity in a ribonucleotide reductase: dGTP-ADP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.035 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.120, 122.860, 106.060 |
| Unit cell angles | 90.00, 103.46, 90.00 |
Refinement procedure
| Resolution | 22.700 - 2.120 |
| R-factor | 0.20763 |
| Rwork | 0.205 |
| R-free | 0.25572 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xje |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.882 |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.300 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 77677 | |
| <I/σ(I)> | 8.9 | 2.48 |
| Completeness [%] | 97.2 | 96.9 |
| Redundancy | 2.15 | 2.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | PEG8000, sodium acetate, sodium chloride, dithiotreithol, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
