1XJD
Crystal Structure of PKC-theta complexed with Staurosporine at 2A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.566, 42.400, 67.678 |
| Unit cell angles | 90.00, 116.24, 90.00 |
Refinement procedure
| Resolution | 19.930 - 2.000 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1stc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.058 | 0.276 |
| Number of reflections | 23134 | |
| <I/σ(I)> | 19.3 | 2.5 |
| Completeness [%] | 63.6 | |
| Redundancy | 3.33 | 2.11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 2M Ammonium Sulfate, 40mM DTT, 0.1M bis-tris, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
