1XJC
X-ray crystal structure of MobB protein homolog from Bacillus stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-16 |
| Detector | SBC-3 |
| Wavelength(s) | 0.979295 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 92.889, 92.889, 39.107 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.410 - 2.100 |
| R-factor | 0.1961 |
| Rwork | 0.196 |
| R-free | 0.22490 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.094 | 0.310 |
| Number of reflections | 11599 | |
| <I/σ(I)> | 42.9 | 9.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.5 | 16.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 289 | citric acid, sodium chloride, pH 3.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
