1XIQ
Plasmodium falciparum Nucleoside diphosphate kinase B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.974609 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.593, 112.424, 68.590 |
| Unit cell angles | 90.00, 119.05, 90.00 |
Refinement procedure
| Resolution | 52.700 - 3.050 |
| R-factor | 0.23238 |
| Rwork | 0.229 |
| R-free | 0.28608 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.156 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.705 | 3.210 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.111 | 0.574 |
| Number of reflections | 17326 | |
| <I/σ(I)> | 7.1 | 1.9 |
| Completeness [%] | 99.4 | 99 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | calcium acetate, PEG3350, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | calcium acetate, PEG3350, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
