1XI1
Phi29 DNA polymerase ssDNA complex, monoclinic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9760 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.557, 170.563, 68.813 |
| Unit cell angles | 90.00, 106.99, 90.00 |
Refinement procedure
| Resolution | 19.710 - 2.200 |
| R-factor | 0.243 |
| Rwork | 0.243 |
| R-free | 0.27700 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.097 | |
| Number of reflections | 66719 | |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 99.1 | 94.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Magnesium acetate, Tris-HCl, salt, ammonium sulfate, PEG 8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Magnesium acetate | ||
| 10 | 1 | 2 | PEG 8000 | ||
| 2 | 1 | 1 | Tris-HCl | ||
| 3 | 1 | 1 | salt | ||
| 4 | 1 | 1 | ammonium sulfate | ||
| 5 | 1 | 1 | PEG 8000 | ||
| 6 | 1 | 2 | Magnesium acetate | ||
| 7 | 1 | 2 | Tris-HCl | ||
| 8 | 1 | 2 | salt | ||
| 9 | 1 | 2 | ammonium sulfate |
