1XHZ
Phi29 DNA polymerase, orthorhombic crystal form, ssDNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9760 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 96.662, 150.397, 198.325 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 2.700 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.870 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.094 | |
| Number of reflections | 78039 | |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 97.7 | 90.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | Magnesium Acetate, Tris-HCl, PEG 8000, Ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Magnesium Acetate | ||
| 2 | 1 | 1 | Tris-HCl | ||
| 3 | 1 | 1 | PEG 8000 | ||
| 4 | 1 | 1 | Ammonium sulfate | ||
| 5 | 1 | 2 | Magnesium Acetate | ||
| 6 | 1 | 2 | Tris-HCl | ||
| 7 | 1 | 2 | PEG 8000 | ||
| 8 | 1 | 2 | Ammonium sulfate |
