1XHK
Crystal structure of M. jannaschii Lon proteolytic domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000, 0.9780, 0.9785, 0.9940 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 89.846, 89.846, 100.609 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.180 - 1.900 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.26300 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 33223 | |
| <I/σ(I)> | 29.8 | 3.1 |
| Completeness [%] | 99.9 | 98.4 |
| Redundancy | 5.9 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
