1XHF
Crystal structure of the bef3-activated receiver domain of redox response regulator arca
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97176 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 86.169, 86.169, 213.082 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.152 |
| R-factor | 0.18978 |
| Rwork | 0.187 |
| R-free | 0.21219 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Model Of ArcA Receiver Domain Without Mg2+/BeF3- |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.445 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.2) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 26280 | |
| <I/σ(I)> | 3.7 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.5 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | Ammonium Acetate, MPD, Sodium Acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
