1XH2

Structure of the N298S variant of human pancreatic alpha-amylase complexed with chloride and acarbose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2003-07-24
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	53.135, 69.392, 132.447
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
Rwork	0.156
R-free	0.21400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bsi
RMSD bond length	0.007
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.200
Rmerge	0.080
Number of reflections	25669
<I/σ(I)>	16.7
Completeness [%]	97.0
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	MPD, cacodylate , pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K