1XGY

Crystal Structure of Anti-Meta I Rhodopsin Fab Fragment K42-41L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-02-28
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	109.721, 196.646, 44.173
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.890 - 2.710
R-factor	0.233
Rwork	0.233
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1N6Q 1fai
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	COMO
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	98.320	2.830
High resolution limit [Å]	2.710	2.710
Rmerge		0.234
Number of reflections	21152
<I/σ(I)>	12.7	2.4
Completeness [%]	78.2	43.2
Redundancy	4.2	1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	peg mme 5000, MES, Ammonium Sulfate, Peptide TGALQERSK, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K