1XGS
METHIONINE AMINOPEPTIDASE FROM HYPERTHERMOPHILE PYROCOCCUS FURIOSUS
Experimental procedure
| Source type | ROTATING ANODE |
| Temperature [K] | 289 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-12 |
| Detector | MAC Science DIP-2030 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.903, 83.286, 70.267 |
| Unit cell angles | 90.00, 105.87, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.750 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.23500 |
| Structure solution method | RIGID-BODY REFINEMENT |
| Starting model (for MR) | STRUCTURE OF NATIVE MONOCLINIC PFMAP CRYSTAL DETERMINED BY MIR METHOD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 24.320 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.075 | 0.361 |
| Total number of observations | 192734 * | |
| Number of reflections | 49955 | |
| <I/σ(I)> | 9.7 | 1.6 |
| Completeness [%] | 84.6 | 62.4 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 4.5 * | 293 * | Tahirov, T., (1998) J. Struct. Biol., 121, 68. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 2 (mM) | ||
| 3 | 1 | drop | potassium acetate | 20 (mM) | |
| 4 | 1 | reservoir | ethanol | 20 (%) | |
| 5 | 1 | reservoir | Tris-HCl | 0.1 (M) |
