1XG8
Crystal Structure of Protein of Unknown Function SA0789 from Staphylococcus aureus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-16 |
| Detector | SBC-2 |
| Wavelength(s) | 0.979569, 0.979706, 0.964107 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 41.174, 41.174, 127.709 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.600 - 2.100 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.28300 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.083 | 0.481 |
| Number of reflections | 11783 | |
| <I/σ(I)> | 18.8 | 1.73 |
| Completeness [%] | 96.0 | 71.8 |
| Redundancy | 7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 273 | ammonium sulfate, citrate buffer, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 273K |
