1XFK

1.8A crystal structure of formiminoglutamase from Vibrio cholerae O1 biovar eltor str. N16961

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-06
Detector	SBC-2
Wavelength(s)	0.9795, 0.9798, 0.94656
Spacegroup name	H 3 2
Unit cell lengths	139.278, 139.278, 134.488
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.950 - 1.800
R-factor	0.202
Rwork	0.202
R-free	0.22900
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.300
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.086
Number of reflections	80918
<I/σ(I)>	19.12	2
Completeness [%]	90.5	56.8
Redundancy	5.8	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	298	25% PEg 3350, 0.2M tri-sodium citrate, pH 7.3, VAPOR DIFFUSION, SITTING DROP, temperature 298K