1XF5
Complex HCV core-Fab 19D9D6-Protein L mutant (H74C, Y64W)in space group P21212
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97564 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 129.337, 222.928, 43.624 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.600 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.650 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Number of reflections | 39554 | |
| Completeness [%] | 97.8 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 10.3% MPEG 5K, 0.5M NaCl, 0.2M Tris-HCl, pH 9, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
