1XF2
Structure of Fab DNA-1 complexed with dT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2002-10-01 |
| Detector | BRANDEIS - B4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 170.678, 170.678, 144.667 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.500 - 2.300 |
| R-factor | 0.20354 |
| Rwork | 0.202 |
| R-free | 0.24194 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i8m |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.402 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 0.446 |
| Number of reflections | 55433 | |
| <I/σ(I)> | 31 | 4.7 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | ammonium sulfate, sodium phosphate, sodium citrate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium sulfate | ||
| 2 | 1 | 1 | sodium phosphate | ||
| 3 | 1 | 1 | sodium citrate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | ammonium sulfate | ||
| 6 | 1 | 2 | sodium phosphate | ||
| 7 | 1 | 2 | sodium citrate |
