1XEX
Structural biochemistry of ATP-driven dimerization and DNA stimulated activation of SMC ATPases.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97547 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 102.600, 102.600, 87.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| Rwork | 0.207 |
| R-free | 0.26100 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 18600 | |
| Completeness [%] | 99.5 | 95.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Na-Acetate, PEG400, ethanol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
