1XEU
Crystal Structure of Internalin C from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 59.327, 177.802, 42.222 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.760 - 2.050 |
| R-factor | 0.2023 |
| Rwork | 0.200 |
| R-free | 0.24256 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | truncated model of Internalin B |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.644 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.091 | 0.484 |
| Number of reflections | 24878 | |
| <I/σ(I)> | 16.3 | 2.2 |
| Completeness [%] | 87.7 | 77.2 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | magnesium sulfate and MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
