1XEO

High Resolution Crystals Structure of Cobalt- Peptide Deformylase Bound To Formate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	93
Detector technology	CCD
Collection date	2000-12-09
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	P 61 2 2
Unit cell lengths	54.420, 54.420, 224.430
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.940 - 1.300
R-factor	0.2
Rwork	0.200
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.350
High resolution limit [Å]	1.300	1.300
Rmerge	0.071	0.239
Number of reflections	48707
<I/σ(I)>	28.17	3.97
Completeness [%]	98.2	86.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	277	Sodium Acetate, Sodium Formate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K