1XEK

THE CRYSTAL STRUCTURES OF LYSOZYME AT VERY LOW LEVELS OF HYDRATION

Experimental procedure

Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1993-12-03
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	25.318, 54.729, 30.681
Unit cell angles	90.00, 111.15, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.197 *
Rwork	0.197
R-free	0.30400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	88% LOW HUMIDITY STRUCTURE
RMSD bond length	0.012
RMSD bond angle	0.047
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.362
High resolution limit [Å]	2.300	2.265
Rmerge	0.041	0.247
Total number of observations	5932 *
Number of reflections	3175
<I/σ(I)>	29.55	3.146
Completeness [%]	86.0	32.4
Redundancy	1.87	1.13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6		pH 4.6