1XEB
Crystal Structure of an Acyl-CoA N-acyltransferase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-20 |
| Detector | SBC-2 |
| Wavelength(s) | 0.979398 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 138.397, 138.397, 136.486 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.890 - 2.350 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.28200 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.078 | 0.389 |
| Number of reflections | 121434 | |
| <I/σ(I)> | 16.5 | 2.7 |
| Completeness [%] | 98.2 | |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | NH4Cl, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
