1XE8
Crystal structure of the YML079w protein from Saccharomyces cerevisiae reveals a new sequence family of the jelly roll fold.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.93 |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 207.970, 207.970, 207.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.800 |
Rwork | 0.223 |
R-free | 0.28440 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.462 |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 19176 | |
<I/σ(I)> | 22 | 6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 20.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 292 | 20-24% PEG 4000, 0.1M Na-Citrate, 0.2M Ammonium Acetate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 292K |