1XDZ
Crystal Structure of Gram_Positive Bacillus subtilis Glucose inhibited Division protein B (gidB), Structural genomics, MCSG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-16 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97835 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.496, 58.164, 51.207 |
| Unit cell angles | 90.00, 123.40, 90.00 |
Refinement procedure
| Resolution | 45.690 - 1.600 |
| R-factor | 0.20306 |
| Rwork | 0.202 |
| R-free | 0.22052 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.237 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.076 | 0.407 |
| Number of reflections | 27352 | |
| <I/σ(I)> | 28.6 | 1.52 |
| Completeness [%] | 94.9 | 61.3 |
| Redundancy | 9.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.2M Mg chloride, 0.1M tris hydrochloride, 25% PEG 3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
