1XDY
Structural and Biochemical Identification of a Novel Bacterial Oxidoreductase, W-containing cofactor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.842, 177.513, 104.214 |
| Unit cell angles | 90.00, 109.73, 90.00 |
Refinement procedure
| Resolution | 38.960 - 2.200 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xdq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.700 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.310 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 146127 | |
| Completeness [%] | 99.3 | 98.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | PEG3350, Magnesium formate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
