1XDV

Experimentally Phased Structure of Human the Son of Sevenless protein at 4.1 Ang.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Collection date	1999-12-15
Wavelength(s)	0.98800, 0.97935, 0.97919, 0.96864
Spacegroup name	P 21 21 21
Unit cell lengths	80.033, 124.710, 245.384
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 4.100
R-factor	0.45
Rwork	0.433
R-free	0.44900
Structure solution method	rigid body refinement of previously determined domains positioned in MAD map.
Starting model (for MR)	1DBH and 1BKD
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.400
High resolution limit [Å]	4.100
Number of reflections	19738
Completeness [%]	96.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	PEG 4000, HEPES, pH 7.5, VAPOR DIFFUSION, temperature 298K