1XDQ
Structural and Biochemical Identification of a Novel Bacterial Oxidoreductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-19 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97965 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 110.345, 165.064, 181.447 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.750 - 2.550 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.27400 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 51827 | |
| Completeness [%] | 96.5 | 82.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 291 | Li2SO4, TEA, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
