1XDL
Structure of human aldolase B associated with hereditary fructose intolerance (A149P), at 277K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2001-07-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 153.483, 153.513, 186.479 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.150 - 3.000 |
| R-factor | 0.308 |
| Rwork | 0.304 |
| R-free | 0.34900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qo5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 91423 | |
| <I/σ(I)> | 18.7 | 2.6 |
| Completeness [%] | 94.9 | 90.2 |
| Redundancy | 4.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | ammonium sulfate, 1,8-diaminooctane, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
