1XDI
Crystal structure of LpdA (Rv3303c) from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-22 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 244.200, 244.200, 104.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.810 |
| R-factor | 0.1944 |
| Rwork | 0.194 |
| R-free | 0.27630 |
| Structure solution method | MIR |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.220 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.102 | 0.249 |
| Number of reflections | 29136 | |
| <I/σ(I)> | 18.3 | 5.1 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 8.6 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | Peg 6000, Tri-sodium Citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
