1XD8
Crystal Structure of the Nitrogenase Fe protein Asp39Asn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-09-08 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.235, 92.109, 62.821 |
| Unit cell angles | 90.00, 101.05, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.227 |
| Rwork | 0.223 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nip |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.773 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.064 | 0.315 |
| Number of reflections | 17701 | |
| <I/σ(I)> | 17.8 | 2 |
| Completeness [%] | 96.4 | 96.5 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 8.5 | 298 | PEG4000, Tris HCl, sodium acetate, pH 8.5, SMALL TUBES, temperature 298K |
