1XD4
Crystal structure of the DH-PH-cat module of Son of Sevenless (SOS)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0781 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.470, 127.360, 279.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 11.990 - 3.640 |
| R-factor | 0.307 |
| Rwork | 0.307 |
| R-free | 0.37100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entris 1DBH 1bkd 1nvv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.750 |
| High resolution limit [Å] | 3.620 | 3.620 |
| Rmerge | 0.063 | 0.631 |
| Number of reflections | 29501 | |
| <I/σ(I)> | 20.6 | 2.2 |
| Completeness [%] | 98.1 | 90.9 |
| Redundancy | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG200, ethylene glycol, strontium chloride, HEPES, sucrose, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
