1XCR
Crystal Structure of Longer Splice Variant of PTD012 from Homo sapiens reveals a novel Zinc-containing fold
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-02-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.572, 52.888, 124.485 |
| Unit cell angles | 90.00, 88.89, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| Rwork | 0.190 |
| R-free | 0.22159 |
| Structure solution method | SAD |
| Starting model (for MR) | NIL |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.310 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.055 | 0.215 |
| Number of reflections | 61064 | |
| <I/σ(I)> | 22.2 | 6.8 |
| Completeness [%] | 95.6 | 95.7 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 25% PEG4000, 200mm ammonium acetate, 12% Glycerol, 100mm Na-acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
