1XCM
Crystal structure of the GppNHp-bound H-Ras G60A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 34.292, 81.419, 121.302 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.610 - 1.840 |
| R-factor | 0.18564 |
| Rwork | 0.183 |
| R-free | 0.24356 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5p21 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.557 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Number of reflections | 14668 | |
| <I/σ(I)> | 15.5 | 2.9 |
| Completeness [%] | 97.5 | 98.9 |
| Redundancy | 4.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG 2000 MME, magnesium sulfate, HEPES, hydrogen peroxide, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
