1XCA
APO-CELLULAR RETINOIC ACID BINDING PROTEIN II
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-04-15 |
| Detector | SIEMENS |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.580, 62.270, 35.120 |
| Unit cell angles | 106.41, 90.69, 110.64 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1CBS WITH LIGAND AND SOLVENT MOLECULES REMOVED |
| RMSD bond length | 0.007 |
| RMSD bond angle | 27.800 * |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.080 | |
| Total number of observations | 32588 * | |
| Number of reflections | 13213 | |
| <I/σ(I)> | 10.4 | 1.5 |
| Completeness [%] | 81.9 | 55.9 |
| Redundancy | 2.75 | 1.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | PROTEIN CONC, 30MG/ML BUFFER 0.1M TRIS, PH 8.0 SALT 0.2M SODIUM ACETATE PRECIPITANT 30% PEG 8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 100 (mM) | |
| 3 | 1 | drop | sodium acetate | 100 (mM) | |
| 4 | 1 | drop | PEG8000 | 15 (%) | |
| 5 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 6 | 1 | reservoir | sodium acetate | 200 (mM) | |
| 7 | 1 | reservoir | PEG8000 | 30 (%) |
