1XBW
1.9A Crystal Structure of the protein isdG from Staphylococcus aureus aureus, Structural genomics, MCSG
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-22 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9795, 0.9798, 0.94656 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.742, 66.779, 67.678 |
| Unit cell angles | 90.00, 105.18, 90.00 |
Refinement procedure
| Resolution | 32.660 - 1.900 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.28500 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.076 | 0.252 |
| Number of reflections | 66916 | |
| <I/σ(I)> | 29.24 | 4.02 |
| Completeness [%] | 93.2 | 61.9 |
| Redundancy | 6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 30% PEG, 0.1M Bis-tris, 0.2M NH4SO4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
