1XBU

Streptomyces griseus aminopeptidase complexed with p-iodo-D-phenylalanine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	95
Detector technology	CCD
Collection date	2001-07-22
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97900
Spacegroup name	P 41 21 2
Unit cell lengths	60.970, 60.970, 144.519
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.200
Rwork	0.127
R-free	0.16700
Structure solution method	Difference fourier from previously determined, related structure
RMSD bond length	0.013
RMSD bond angle	0.029
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.220
High resolution limit [Å]	1.200	1.200
Number of reflections	80710
<I/σ(I)>	8.6
Completeness [%]	93.6	63.2
Redundancy	10	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	PEG 4000, sodium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K