1XBS

Crystal structure of human dim2: a dim1-like protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	2000-06-23
Detector	MARRESEARCH
Wavelength(s)	1.08
Spacegroup name	P 41 21 2
Unit cell lengths	78.160, 78.160, 60.334
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.41
Rwork	0.242
R-free	0.30500
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.500	2.500
Number of reflections	6538
Completeness [%]	89.9	93.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	Ammonium sulfate, sodium chloride, EDTA, Tris-HCl, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K