1XBR
T DOMAIN FROM XENOPUS LAEVIS BOUND TO DNA
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.900, 113.900, 149.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.28300 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 24.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.136 * | |
| Total number of observations | 101653 * | |
| Number of reflections | 22983 | |
| <I/σ(I)> | 33.5 | 13.7 |
| Completeness [%] | 98.9 | 98.9 |
| Redundancy | 4.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | PROTEIN WAS CRYSTALLIZED FROM 2-8% PEG 8K, 20 MM MGCL2 50 MM NA-ACETATE, PH 4.2 1MM DTT, 5 MM SPERMINE, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 10 | 1 | 2 | SPERMINE | ||
| 2 | 1 | 1 | NACL | ||
| 3 | 1 | 1 | HEPES | ||
| 4 | 1 | 1 | DTT | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | PEG 8000 | ||
| 7 | 1 | 2 | MGCL2 | ||
| 8 | 1 | 2 | NA ACETATE | ||
| 9 | 1 | 2 | DTT |
