1XBN

Crystal structure of a bacterial nitric oxide sensor: an ortholog of mammalian soluble guanylate cyclase heme domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-03
Detector	ADSC QUANTUM 315
Wavelength(s)	1.7403,1.7379,1.6241,1.1159
Spacegroup name	P 42 3 2
Unit cell lengths	120.607, 120.607, 120.607
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	84.510 - 2.500
R-factor	0.26471
Rwork	0.263
R-free	0.29147
Structure solution method	MAD
RMSD bond length	0.023
RMSD bond angle	1.991
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.000	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	10884
<I/σ(I)>	41	2.5
Completeness [%]	99.8	100
Redundancy	11	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	277	MPD, cacodylate, pH 6, VAPOR DIFFUSION, temperature 277K