1XBI
High resolution structure of Methanocaldococcus jannaschii L7AE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.665, 48.869, 51.914 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.630 - 1.450 |
| R-factor | 0.13533 |
| Rwork | 0.132 |
| R-free | 0.19296 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ra4 |
| RMSD bond length | 0.063 |
| RMSD bond angle | 4.590 |
| Data reduction software | MADNESS |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.580 | 1.488 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Number of reflections | 19412 | |
| <I/σ(I)> | 31.5044 | 3.195 |
| Completeness [%] | 100.0 | 70.4 |
| Redundancy | 7.1 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% PEG 3350, 50mM HEPES, 10% isopropanol, 5% ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
