1XBC
Crystal structure of the syk tyrosine kinase domain with Staurosporin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Detector technology | CCD |
| Collection date | 2003-11-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.324, 84.650, 90.904 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.400 - 2.000 |
| Rwork | 0.246 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.547 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.513 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.112 | 0.016 |
| Number of reflections | 20908 | |
| <I/σ(I)> | 10.1 | 1.6 |
| Completeness [%] | 96.0 | 80.2 |
| Redundancy | 6.3 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 15% peg 3350, 100mM Tris HCl (pH 8.5) |
