1XAS

CRYSTAL STRUCTURE, AT 2.6 ANGSTROMS RESOLUTION, OF THE STREPTOMYCES LIVIDANS XYLANASE A, A MEMBER OF THE F FAMILY OF BETA-1,4-D-GLYCANSES

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	69.520, 46.510, 85.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.600
R-factor	0.21
Rwork	0.210
RMSD bond length	0.011
RMSD bond angle	0.045 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *
High resolution limit [Å]	2.590 *	2.590 *
Rmerge	0.082 *
Number of reflections	8479 *
Completeness [%]	92.9 *	56.1 *
Redundancy	8.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	60 (mg/ml)
2	1	drop	PEG4000	5 (%)
3	1	drop	sodium acetate	100 (mM)
4	1	reservoir	PEG4000	5 (%)
5	1	reservoir	sodium acetate	100 (mM)