1XAF
Crystal Structure of Protein of Unknown Function YfiH from Shigella flexneri 2a str. 2457T
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-17 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.874, 50.583, 55.347 |
| Unit cell angles | 90.32, 96.32, 90.40 |
Refinement procedure
| Resolution | 55.050 - 2.010 |
| R-factor | 0.18583 |
| Rwork | 0.179 |
| R-free | 0.25159 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.517 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.050 | 2.080 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.071 | 0.206 |
| Number of reflections | 30856 | |
| <I/σ(I)> | 11.3 | 6.5 |
| Completeness [%] | 97.4 | 89.6 |
| Redundancy | 4.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | PEG 3350, Zinc acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
