1X9P

The crystal structure of human adenovirus 2 penton base

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-19
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	C 1 2 1
Unit cell lengths	435.984, 300.168, 420.621
Unit cell angles	90.00, 104.36, 90.00

Refinement procedure

Resolution	20.000 - 3.300
R-factor	0.307
Rwork	0.307
R-free	0.30700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	electron microscopy map of ad3 dodecahedron
RMSD bond length	0.012
RMSD bond angle	1.900
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.390
High resolution limit [Å]	3.300	3.300
Rmerge	0.210	0.470
Number of reflections	447016
<I/σ(I)>	2.9	1.6
Completeness [%]	57.3	22
Redundancy	1.5	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	288	1.6M Ammonium sulfate, 10% dioxane, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K
1	VAPOR DIFFUSION, HANGING DROP	6.5	288	1.6M Ammonium sulfate, 10% dioxane, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K
1	VAPOR DIFFUSION, HANGING DROP	6.5	288	1.6M Ammonium sulfate, 10% dioxane, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K