1X9K
An all-RNA Hairpin Ribozyme with mutation U39C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-17 |
Detector | MARRESEARCH |
Wavelength(s) | 1.00 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 94.460, 94.460, 129.120 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.630 - 3.170 |
R-factor | 0.236 |
Rwork | 0.236 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m5k |
RMSD bond length | 0.009 |
RMSD bond angle | 1.600 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | CrystalClear ((MSC/RIGAKU)) |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.280 |
High resolution limit [Å] | 3.170 | 3.170 |
Number of reflections | 6480 | |
<I/σ(I)> | 13.4 | 3.9 |
Completeness [%] | 98.3 | 100 |
Redundancy | 6.6 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | ammonium sulfate, spermidine, cobalt hexaamine, cacodylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | ammonium sulfate | ||
2 | 1 | 1 | spermidine | ||
3 | 1 | 1 | cobalt hexaamine | ||
4 | 1 | 1 | cacodylate | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | ammonium sulfate | ||
7 | 1 | 2 | cacodylate | ||
8 | 1 | 2 | H2O |