1X8W
Structure of the Tetrahymena Ribozyme: Base Triple Sandwich and Metal Ion at the Active Site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 175.400, 175.400, 304.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.800 |
| R-factor | 0.267 |
| Rwork | 0.263 |
| R-free | 0.32000 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.970 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.054 | 0.255 |
| Number of reflections | 43972 | |
| <I/σ(I)> | 24 | 3.6 |
| Completeness [%] | 93.9 | 76.8 |
| Redundancy | 4.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 303 | 9.5% 2-methyl-2,4-pentanediol (MPD), 25 mM K cacodylate pH 6.0, 25 mM KCl, 5mM NaCl, 17.5 mM MgCl2 and 0.25 mM spermine, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 10 | 1 | 2 | NaCl | ||
| 11 | 1 | 2 | MgCl2 | ||
| 12 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | cacodylate | ||
| 3 | 1 | 1 | KCl | ||
| 4 | 1 | 1 | NaCl | ||
| 5 | 1 | 1 | MgCl2 | ||
| 6 | 1 | 1 | spermine | ||
| 7 | 1 | 1 | H2O | ||
| 8 | 1 | 2 | cacodylate | ||
| 9 | 1 | 2 | KCl |
