1X8R
EPSPS liganded with the (S)-phosphonate analog of the tetrahedral reaction intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-11 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.730, 85.170, 87.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.500 |
| R-factor | 0.168 |
| Rwork | 0.168 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g6s |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.560 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.048 | 0.143 |
| Number of reflections | 66430 | |
| <I/σ(I)> | 18.3 | 7.1 |
| Completeness [%] | 95.3 | 92.1 |
| Redundancy | 6.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | Na-formate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
